Protein Interactions

This volume, part of the Protein Reviews book series, will successfully and clearly examine how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It will deal with the methodology behind the research and how to synergistically incorporate several methodologies for use. The study of complex systems cannot be described or summarized in the abstract; therefore, each chapter would treat and introduce the reader to different biological systems, include a brief summary of the physical principles, and mention practical requirements (such as amount of material required), but not be of recipe type, and instead rapidly lead to principles and examples of applications to complex systems. TOC:The Characterization of Bio-molecular Interactions Using Fluorescence Fluctuation Techniques.- Characterization of Protein-protein Interactions Using Atomic Force Microscopy.- Combined Solid Phase Detection Techniques for Dissecting Multi-Protein Interactions on Membranes.- Surface Plasmon Resonance Biosensing in the Study of Ternary Systems of Interacting Proteins.- Mass Spectrometry for Studying Protein Modifications and for Discovery of Protein Interactions.- H/2H Exchange Mass Spectrometry of Protein Complexes.- Elucidation of Protein-Protein and Protein-Ligand Interactions by NMR-Spectroscopy.- Application of Isothermal Titration Calorimetry in Exploring the Extended Interface.- Solvent Mediated Protein-Protein Interactions.- Sedimentation Equilibrium Analytical Ultracentrifugation for Multi-Component Protein Interactions.- Structure analysis of macromolecular complexes by solution small-angle scattering.- Fluorescence Detection of Proximity.- Steady state and Time-resolved Emission Anisotropy.- Analysis of Protein-DNA Equilibria By Native Gel Electrophoresis.- Electrospray Ionisation Mass Spectrometry and the Study of Protein Complexes.- Sedimentation velocity in the study of reversible multi-protein complexes.

Tytuł: Protein Interactions
Seria: Protein Reviews
Data wydania: marzec 2007
Wydawca: Springer-Verlag GmbH
ISBN: 0387359656
EAN: 9780387359656
Wymiary: 245 x 160 x 37